Outreach

This project will perform single nucleus RNA sequencing (snRNA-seq) on three brain regions with the possibility of identifying gene targets that exhibit region and cell type specific selectivity of opioid use disorder in the context of HIV/HIV associated neurocognitive impairment.

This project will perform snRNA-seq, single-nucleus ATAC-seq (scATAC-seq), and spatial transcriptomics on 20 brains from four donor groups: controls, HIV (HIV+), HIV with OUD (HIV+OUD+), and OUD without HIV (OUD+). Three regions which represent disease-relevant areas for OUD and HIV, will be assayed: prefrontal cortex, ventral striatum and insular cortex.

This project will engage in mapping cell specific transcriptomes in monoaminergic circuitry associated with prospectively monitored neurological status in the years before death and exposure to drug of abuse. It will also explore whether or not opiate and cocaine exposures affect HIV neurogenomic activity.

This project will generate a single-cell transcriptome and regulome atlas of gene regulatory networks in the human brain during neuroimmunologic perturbations by methamphetamine and HIV infection.

This project will leverage state-of-the-art single cell and single nuclei RNA sequencing combined with molecular, cellular and behavioral approaches to define cell type-specific interactions between HIV and opioids in the brains of EcoHIV-infected mice.

This project will perform studies on the interplay of HIV pathogenesis and opioid abuse in the gold-standard SIV/nonhuman primate system, using single-cell RNA sequencing (scRNAseq) to assess individual cellular transcriptomes.

This project will dissect the transcriptional and epigenetic changes associated with SUD and persistent HIV infection at single-cell resolution across seven human brain regions, and integrate the resulting datasets to predict driver genes.

This project puts forward an unprecedented concept and experimental system to identify single cell determinants of the brain relevant to persistent HIV infection and opioid use disorder as well as potential opportunities to illuminate how genetic variation affects gene expression.

This projects seeks to generate topographical data sets and evidence at single cell resolution across the hippocampus and prefrontal cortex (PFC), two brain regions known for predilection for HIV persistence and OUD in non-human primate (NHP) and in post-mortem human brain. These data will provide an unprecedented cellular landscape of multiple modalities that can be harnessed to develop strategies to limit viral persistence and restore and retain optimal brain health in people living with HIV.